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KMID : 1134820150440050716
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Journal of the Korean Society of Food Science and Nutrition
2015 Volume.44 No. 5 p.716 ~ p.724
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Validation of Trienzyme Extraction-Microplate Assay for Folate in Korean Ancestral Rite Food
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Park Su-Jin
Jeong Beom-Gyun
Jung Jae-Eun
Kim Hyeon-Young
Jung Gil-Rak
Hwang Eun-Jung
Yoon Sung-Won
Hyun Tai-Sun
Lee Jun-Soo
Chun Ji-Yeon
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Abstract
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Trienzyme extraction coupled with microplate assay (Lactobacillus casei subsp. rhamnosus) was validated and applied for the determination of folate (vitamin B9) in Korean ancestral rite foods. Foods included five Guk (Tang), eleven Sookchaes, eight Jeoks, nine Jeons, six Jjims, and twenty desserts. Folate was detected in all samples: Guk (Tang) 4.62∼18.84, Sookchae 6.13∼48.40, Jeok 5.49∼49.50, Jeon 6.96∼30.77, Jjim 10.34∼38.88, and desserts 3.33∼49.55 μg/100 g. The lowest folate content was observed in Sikhye (3.33 μg/100 g), whereas the highest was observed in Songhwa-dasik (49.55 μg/100 g). Folate analyses of certified reference materials, BCR-121 (whole meal flour) and BCR-487 (pig liver), showed good recoveries of 90.0% (0.45 mg/kg) and 92.4% (12.3 mg/kg), respectively. The recoveries (96.0 to 106.2%) obtained by analyzing eight spiked samples with different matrices also showed good accuracy. Both repeatability and reproducibility were less than 5%, indicating good precision. The quality control chart (n>30) obtained by running commercial folate fortified-wheat flour once a week for about 10 months showed that all assays were under control. All validation method and analytical quality control results showed that folate contents in Korean ancestral rite foods produced by microplate assay were reliable enough to be used for the construction of a national folate database.
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KEYWORD
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folate, trienzyme extraction, microplate assay, Korean ancestral rite food, method validation
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